For genome, the annotation file could be a .tab file with the following fields (header is not needed in the .tab file):
Gene name |
Chromosome |
Strand |
CDS Length |
Number of CDS regions |
CDS Start 1 |
CDS End 1 |
CDS Start 2 |
CDS End 2 |
YAL008W |
chrI |
+ |
597 |
1 |
136914 |
137510 |
|
|
YBL087C |
chrII |
- |
414 |
2 |
59822 |
60193 |
60698 |
60739 |
Alternatively, a GFF file can also be given as input for annotations, but the script is optimized for processing GFF files for E. coli and S. cerevisiae only.